LentiCRISPRv2-ATG5
(Plasmid #99573)

• Purpose: Expresses Cas9 with sgRNA targeting Exon 7 of ATG5
• Depositing Lab: Edward Campbell
• Publication: Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Backbone

• Vector backbone: LentiCRISPR V2
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gRNA targeting ATG5
• gRNA/shRNA sequence: CACCGGATGGACAGTTGCACACACT
• Species: H. sapiens (human)
• Entrez Gene: ATG5 (a.k.a. APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry