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Addgene

p415TEF-NYFP
(Plasmid #99555)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99555 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p415TEF1
  • Backbone manufacturer
    ATCC
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Swi1 N-rich region (AA1-327)
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    SWI1 (a.k.a. YPL016W, ADR6, GAM3, LPA1)
  • Promoter TEF1
  • Tag / Fusion Protein
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TEF1
  • 3′ sequencing primer CYCter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p415TEF-NYFP was a gift from Liming Li (Addgene plasmid # 99555 ; http://n2t.net/addgene:99555 ; RRID:Addgene_99555)

  • For your References section:

    [SWI], the prion formed by the chromatin remodeling factor Swi1, is highly sensitive to alterations in Hsp70 chaperone system activity. Hines JK, Li X, Du Z, Higurashi T, Li L, Craig EA. PLoS Genet. 2011 Feb;7(2):e1001309. doi: 10.1371/journal.pgen.1001309. Epub 2011 Feb 17. 10.1371/journal.pgen.1001309 PubMed 21379326
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