p415TEF-NQYFP
(Plasmid
#99554)
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PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep415TEF1
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Backbone manufacturerATCC
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSwi1 NQ-rich region (AA1-536)
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneSWI1 (a.k.a. YPL016W, ADR6, GAM3, LPA1)
- Promoter TEF1
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Tag
/ Fusion Protein
- YFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer TEF1
- 3′ sequencing primer CYCter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p415TEF-NQYFP was a gift from Liming Li (Addgene plasmid # 99554 ; http://n2t.net/addgene:99554 ; RRID:Addgene_99554) -
For your References section:
Distinct subregions of Swi1 manifest striking differences in prion transmission and SWI/SNF function. Du Z, Crow ET, Kang HS, Li L. Mol Cell Biol. 2010 Oct;30(19):4644-55. doi: 10.1128/MCB.00225-10. Epub 2010 Aug 2. 10.1128/MCB.00225-10 PubMed 20679490