Skip to main content
Addgene

His6tev CBP BCHZDeltaAL
(Plasmid #99551)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 7433
  • Modifications to backbone
    introduction of His6tev N-terminal tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mouse CBP BRD-ZZ domains (1079-1751) DeltaAL
  • Alt name
    crebbp
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1940
  • Mutation
    replaced 1557-1618 with SGGSG
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Promoter T7
  • Tag / Fusion Protein
    • His6tev (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6tev CBP BCHZDeltaAL was a gift from Peter Wright (Addgene plasmid # 99551 ; http://n2t.net/addgene:99551 ; RRID:Addgene_99551)

  • For your References section:

    Role of the CBP catalytic core in intramolecular SUMOylation and control of histone H3 acetylation. Park S, Stanfield RL, Martinez-Yamout MA, Dyson HJ, Wilson IA, Wright PE. Proc Natl Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 10.1073/pnas.1703105114. 10.1073/pnas.1703105114 PubMed 28630323
Related items:
Commonly requested with: