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Addgene

YIp128-P30-His-pol30(K127R)
(Plasmid #99543)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99543 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YIplac128
  • Backbone manufacturer
    Gietz & Sugino (1988) Gene 74:527-34
  • Backbone size w/o insert (bp) 4293
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POL30
  • Alt name
    PCNA
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1569
  • Mutation
    K127R
  • Entrez Gene
    POL30 (a.k.a. YBR088C)
  • Promoter POL30
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer M13fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ORF is followed by the ADH1 transcription terminator; digest with BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIp128-P30-His-pol30(K127R) was a gift from Helle Ulrich (Addgene plasmid # 99543 ; http://n2t.net/addgene:99543 ; RRID:Addgene_99543)

  • For your References section:

    Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD. Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36. 10.1007/978-1-61779-998-3_36 PubMed 22941627
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