YEp181-CUP1-His-Ubi
(Plasmid
#99539)
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PurposeYeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99539 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneYEplac181
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Backbone manufacturerGietz & Sugino (1988) Gene 74:527-34
- Backbone size w/o insert (bp) 5741
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUBI3(1-76)
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Alt nameubiquitin
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)916
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Entrez GeneRPL40A (a.k.a. YIL148W, CEP52A, UB11, UBI1)
- Promoter CUP1
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Tag
/ Fusion Protein
- 7xHis (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site SphI (unknown if destroyed)
- 5′ sequencing primer M13fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The ORF is followed by the ADH1 transcription terminator.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YEp181-CUP1-His-Ubi was a gift from Helle Ulrich (Addgene plasmid # 99539 ; http://n2t.net/addgene:99539 ; RRID:Addgene_99539) -
For your References section:
Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD. Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36. 10.1007/978-1-61779-998-3_36 PubMed 22941627