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YEp181-CUP1-His-Smt3
(Plasmid #99536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99536 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YEplac181
  • Backbone manufacturer
    Gietz & Sugino (1988) Gene 74:527-34
  • Backbone size w/o insert (bp) 5741
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMT3
  • Alt name
    SUMO
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    991
  • Entrez Gene
    SMT3 (a.k.a. YDR510W)
  • Promoter CUP1
  • Tag / Fusion Protein
    • 7xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer M13fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YEp181-CUP1-His-Smt3 was a gift from Helle Ulrich (Addgene plasmid # 99536 ; http://n2t.net/addgene:99536 ; RRID:Addgene_99536)

  • For your References section:

    In vivo detection and characterization of sumoylation targets in Saccharomyces cerevisiae. Ulrich HD, Davies AA. Methods Mol Biol. 2009;497:81-103. doi: 10.1007/978-1-59745-566-4_6. 10.1007/978-1-59745-566-4_6 PubMed 19107412
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