pMK110
(Plasmid #99535)

• Purpose: contains bgl operon sequence for E. coli in vitro transcription template
• Depositing Lab: Robert Landick
• Publication: Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife.04970.

Backbone

• Vector backbone: pIA267
• Backbone size w/o insert (bp): 4891
• Total vector size (bp): 5886
• Modifications to backbone: None.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E. coli bgl antisense region containing bglG
• Species: E. coli K-12
• Insert Size (bp): 995
• Promoter: lambda Pr promoter used for in vitro transcription template (from plasmid backbone)
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: M13 Fwd
• 3′ sequencing primer: M13 Rev

Resource Information

• Supplemental Documents:
  • pMK110- pIA267+bglRegion Antiparrallel-more annotations.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry