pcDNA3.1(-)-WIF1
(Plasmid
#99533)
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PurposeWIF1 expression plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 (-)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWIF1
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Alt nameWNT inhibitory factor 1
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SpeciesH. sapiens (human)
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Entrez GeneWIF1 (a.k.a. WIF-1)
- Promoter CMV
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(-)-WIF1 was a gift from Qian Tao (Addgene plasmid # 99533 ; http://n2t.net/addgene:99533 ; RRID:Addgene_99533) -
For your References section:
The tumor suppressor Wnt inhibitory factor 1 is frequently methylated in nasopharyngeal and esophageal carcinomas. Chan SL, Cui Y, van Hasselt A, Li H, Srivastava G, Jin H, Ng KM, Wang Y, Lee KY, Tsao GS, Zhong S, Robertson KD, Rha SY, Chan AT, Tao Q. Lab Invest. 2007 Jul;87(7):644-50. Epub 2007 Mar 26. 10.1038/labinvest.3700547 PubMed 17384664