pRS303-PADH1-AFB2
(Plasmid
#99530)
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PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS303
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAFB2
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2804
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Entrez GeneAFB2 (a.k.a. AT3G26810, auxin signaling F-box 2)
- Promoter ADH1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EagI (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe AFB2 gene was a kind gift from Jennifer L. Nemhauser (University of Washington, Seattle, USA).
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Digest vector with NheI for integration into HIS3
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS303-PADH1-AFB2 was a gift from Helle Ulrich (Addgene plasmid # 99530 ; http://n2t.net/addgene:99530 ; RRID:Addgene_99530)
Map uploaded by the depositor.
