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Addgene

pHyg-AID*
(Plasmid #99515)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99515 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a derivative
  • Vector type
    PCR template for yeast tagging cassette
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IAA17(71-114)
  • Alt name
    AID (Auxin-inducible degron)
  • Alt name
    AXR3
  • Species
    A. thaliana (mustard weed)
  • Mutation
    internal inversion of nucleotides 265-312 of the IAA17 ORF, affecting aa89-104
  • Entrez Gene
    AXR3 (a.k.a. AT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer CGTACGCTGCAGGTCGAC
  • 3′ sequencing primer TCGATGAATTCGAGCTCG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The parent vector encoding AID was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Caution: internal inversion within the IAA17 sequence (aa89-104)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHyg-AID* was a gift from Helle Ulrich (Addgene plasmid # 99515 ; http://n2t.net/addgene:99515 ; RRID:Addgene_99515)

  • For your References section:

    An expanded tool kit for the auxin-inducible degron system in budding yeast. Morawska M, Ulrich HD. Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967. Epub 2013 Jul 23. 10.1002/yea.2967 PubMed 23836714
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