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Addgene

MAD2
(Plasmid #99512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIND(SP1)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5081
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    618
  • Entrez Gene
    MAD2L1 (a.k.a. HSMAD2, MAD2)
  • Promoter heat shock

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAD2 was a gift from Kuan-Teh Jeang & Dong-Yan Jin (Addgene plasmid # 99512 ; http://n2t.net/addgene:99512 ; RRID:Addgene_99512)

  • For your References section:

    Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Jin DY, Spencer F, Jeang KT. Cell. 1998 Apr 3;93(1):81-91. S0092-8674(00)81148-4 [pii] PubMed 9546394
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