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Addgene

MIP-T3
(Plasmid #99511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTag2B
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4324
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIP-T3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2073
  • Entrez Gene
    TRAF3IP1 (a.k.a. CFAP116, FAP116, IFT54, MIP-T3, MIPT3, SLSN9)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIP-T3 was a gift from Dong-Yan Jin (Addgene plasmid # 99511 ; http://n2t.net/addgene:99511 ; RRID:Addgene_99511)

  • For your References section:

    MIP-T3 is a negative regulator of innate type I IFN response. Ng MH, Ho TH, Kok KH, Siu KL, Li J, Jin DY. J Immunol. 2011 Dec 15;187(12):6473-82. doi: 10.4049/jimmunol.1100719. Epub 2011 Nov 11. 10.4049/jimmunol.1100719 PubMed 22079989
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