GST-BRD (1079-1198)
(Plasmid
#99507)
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PurposeBacterial expression of mouse CBP bromodomain
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX4T2
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 4970
- Total vector size (bp) 5336
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemouse CBP BRD (1079-1198)
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Alt namecrebbp
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SpeciesM. musculus (mouse)
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Insert Size (bp)366
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Entrez GeneCrebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX
- 3′ sequencing primer pGEX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-BRD (1079-1198) was a gift from Peter Wright (Addgene plasmid # 99507 ; http://n2t.net/addgene:99507 ; RRID:Addgene_99507) -
For your References section:
The CH2 domain of CBP/p300 is a novel zinc finger. Park S, Martinez-Yamout MA, Dyson HJ, Wright PE. FEBS Lett. 2013 Aug 19;587(16):2506-11. doi: 10.1016/j.febslet.2013.06.051. Epub 2013 Jul 4. 10.1016/j.febslet.2013.06.051 PubMed 23831576
