lenti-HA-mBec1 F121A
(Plasmid #99506)

• Purpose: Lentiviral expression of mutant Becn1 leading to hyperactive autophagy
• Depositing Lab: Congcong He
• Publication: Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962. doi: 10.1371/journal.pgen.1006962. eCollection 2017 Aug.

Backbone

• Vector backbone: pCDH-CMV-MCS-EF1-copGFP-T2A-Puro
• Backbone manufacturer: System Biosciences
• Vector type: Lentiviral
• Selectable markers: Puromycin ; copGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Beclin 1
• Alt name: Becn1
• Species: M. musculus (mouse)
• Mutation: F121A
• Entrez Gene: Becn1 (a.k.a. Atg6)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: LNCX

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry