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Addgene

lenti-HA-mBec1
(Plasmid #99505)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99505 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1-copGFP-T2A-Puro
  • Backbone manufacturer
    System Biosciences
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin ; copGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beclin 1
  • Alt name
    Becn1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Becn1 (a.k.a. Atg6)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer LNCX
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-HA-mBec1 was a gift from Congcong He (Addgene plasmid # 99505 ; http://n2t.net/addgene:99505 ; RRID:Addgene_99505)

  • For your References section:

    A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. Rocchi A, Yamamoto S, Ting T, Fan Y, Sadleir K, Wang Y, Zhang W, Huang S, Levine B, Vassar R, He C. PLoS Genet. 2017 Aug 14;13(8):e1006962. doi: 10.1371/journal.pgen.1006962. eCollection 2017 Aug. PGENETICS-D-17-00681 [pii] PubMed 28806762
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