pcDNA-Evf2
(Plasmid
#99478)
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PurposeMammalian expression of the Evf2 long non-coding RNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEvf2
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Alt nameDlx6os
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SpeciesR. norvegicus (rat)
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Insert Size (bp)3874
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-Evf2 was a gift from Jhumku Kohtz (Addgene plasmid # 99478 ; http://n2t.net/addgene:99478 ; RRID:Addgene_99478) -
For your References section:
The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved region and functions as a Dlx-2 transcriptional coactivator. Feng J, Bi C, Clark BS, Mady R, Shah P, Kohtz JD. Genes Dev. 2006 Jun 1;20(11):1470-84. Epub 2006 May 16. 10.1101/gad.1416106 PubMed 16705037