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Addgene

pMH-SFB-RNF8
(Plasmid #99396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMH-SFB
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNF8
  • Species
    H. sapiens (human)
  • Entrez Gene
    RNF8 (a.k.a. hRNF8)
  • Tag / Fusion Protein
    • SFB (S protein-FLAG-Streptavidin binding peptide) (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-SFB-RNF8 was a gift from Junjie Chen & Michael Huen (Addgene plasmid # 99396 ; http://n2t.net/addgene:99396 ; RRID:Addgene_99396)

  • For your References section:

    RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly. Huen MS, Grant R, Manke I, Minn K, Yu X, Yaffe MB, Chen J. Cell. 2007 Nov 30;131(5):901-14. Epub 2007 Nov 20. 10.1016/j.cell.2007.09.041 PubMed 18001825
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