Skip to main content
Addgene

pMH-SFB-BRCA2
(Plasmid #99395)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99395 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMH-SFB
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA2
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRCA2 (a.k.a. BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11)
  • Tag / Fusion Protein
    • SFB (S protein-FLAG-Streptavidin binding peptide) (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The BRCA2 gene in this plasmid contains D2005G, E2175G, and I3286V mutations. The depositor confirms that these have no affect on plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-SFB-BRCA2 was a gift from Junjie Chen & Michael Huen (Addgene plasmid # 99395 ; http://n2t.net/addgene:99395 ; RRID:Addgene_99395)

  • For your References section:

    PALB2 is an integral component of the BRCA complex required for homologous recombination repair. Sy SM, Huen MS, Chen J. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7155-60. doi: 10.1073/pnas.0811159106. Epub 2009 Apr 15. 10.1073/pnas.0811159106 PubMed 19369211
Related items:
Commonly requested with: