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Addgene

pMH-SFB-BRCA1
(Plasmid #99394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMH-SFB
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Tag / Fusion Protein
    • SFB (S protein-FLAG-Streptavidin binding peptide) (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-SFB-BRCA1 was a gift from Junjie Chen & Michael Huen (Addgene plasmid # 99394 ; http://n2t.net/addgene:99394 ; RRID:Addgene_99394)

  • For your References section:

    PALB2 is an integral component of the BRCA complex required for homologous recombination repair. Sy SM, Huen MS, Chen J. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7155-60. doi: 10.1073/pnas.0811159106. Epub 2009 Apr 15. 10.1073/pnas.0811159106 PubMed 19369211
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