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Purpose(Empty Backbone) Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMH-SFB
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Vector typeMammalian Expression
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- SFB (S protein-FLAG-Streptavidin binding peptide) (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- SFB (S protein-FLAG-Streptavidin binding peptide) (N terminal on backbone)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We confirm clones following a LR reaction by RE digestion using EcoR1 or Sal1, which cleaves twice and should release cDNA insert +300bp DNA. The vector is ~7 kb.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-SFB was a gift from Michael Huen (Addgene plasmid # 99391 ; http://n2t.net/addgene:99391 ; RRID:Addgene_99391) -
For your References section:
Dual-utility NLS drives RNF169-dependent DNA damage responses. An L, Jiang Y, Ng HH, Man EP, Chen J, Khoo US, Gong Q, Huen MS. Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2872-E2881. doi: 10.1073/pnas.1616602114. Epub 2017 Mar 21. 10.1073/pnas.1616602114 PubMed 28325877
