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pHub10-His-YFP-PIP2;1
(Plasmid #99386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHub10
  • Backbone manufacturer
    self-made, modified fro pBI121
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PIP2;1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1549
  • Entrez Gene
    PIP2A (a.k.a. AT3G53420, AtPIP2;1, PIP2, PIP2;1, PLASMA MEMBRANE INTRINSIC PROTEIN 2, PLASMA MEMBRANE INTRINSIC PROTEIN 2;1, plasma membrane intrinsic protein 2A)
  • Promoter 2xCaMV35S
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHub10-His-YFP-PIP2;1 was a gift from Ning Li (Addgene plasmid # 99386 ; http://n2t.net/addgene:99386 ; RRID:Addgene_99386)

  • For your References section:

    Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis. Qing D, Yang Z, Li M, Wong WS, Guo G, Liu S, Guo H, Li N. Mol Plant. 2016 Jan 4;9(1):158-74. doi: 10.1016/j.molp.2015.10.001. Epub 2015 Oct 22. 10.1016/j.molp.2015.10.001 PubMed 26476206
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