pLV-TetO-hNGN2-Neo
(Plasmid #99378)

• Purpose: Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.
• Depositing Lab: Kristen Brennand
• Publication: Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27.

Backbone

• Vector backbone: Tet-O-FUW
• Backbone size w/o insert (bp): 8387
• Total vector size (bp): 10108
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hNGN2-P2A-Neo
• Species: H. sapiens (human)
• Entrez Gene: NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
• Promoter: TetO

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: hNGN2: Addgene plasmid #79049 (Kristen Brennand lab)
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry