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Addgene

pCGT-HCF-1-C600
(Plasmid #99364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCGT
  • Backbone size w/o insert (bp) 5068
  • Total vector size (bp) 6959
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCF-1 C-term 1436-2035
  • Alt name
    HCFC1 C-term
  • Alt name
    host cell factor C1 C-term
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1891
  • Mutation
    Deletion of HCFC1 cDNA
  • GenBank ID
    NM_005334.2 NP_005325.2
  • Entrez Gene
    HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 2x tandem T7 tag (N terminal on backbone)
    • C-myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV_for
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGT-HCF-1-C600 was a gift from Winship Herr (Addgene plasmid # 99364 ; http://n2t.net/addgene:99364 ; RRID:Addgene_99364)

  • For your References section:

    HCF-1 amino- and carboxy-terminal subunit association through two separate sets of interaction modules: involvement of fibronectin type 3 repeats. Wilson AC, Boutros M, Johnson KM, Herr W. Mol Cell Biol. 2000 Sep;20(18):6721-30. PubMed 10958670
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