pJC20cdc8.110
(Plasmid
#99363)
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PurposeBacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJC20
- Total vector size (bp) 2807
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecdc8
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)486
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MutationAlanine 18 to Threonine and Glutamic acid 31 to Lysine
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Entrez Genecdc8 (a.k.a. SPAC27F1.02c, fus4)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7F
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC20cdc8.110 was a gift from Dan Mulvihill (Addgene plasmid # 99363 ; http://n2t.net/addgene:99363 ; RRID:Addgene_99363) -
For your References section:
Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on protein stability that destabilise the actin-tropomyosin copolymer in vitro and in vivo. Johnson CA, Brooker HR, Gyamfi I, O'Brien J, Ashley B, Brazier JE, Dean A, Embling J, Grimsey E, Tomlinson AC, Wilson EG, Geeves MA, Mulvihill DP. Biochem Biophys Res Commun. 2017 Oct 25. pii: S0006-291X(17)32087-9. doi: 10.1016/j.bbrc.2017.10.109. 10.1016/j.bbrc.2017.10.109 PubMed 29080743