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Addgene

pJC20cdc8.27
(Plasmid #99362)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC20
  • Total vector size (bp) 2807
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc8
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    486
  • Mutation
    Glutamic acid 129 to Lysine
  • Entrez Gene
    cdc8 (a.k.a. SPAC27F1.02c, fus4)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 F
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC20cdc8.27 was a gift from Dan Mulvihill (Addgene plasmid # 99362 ; http://n2t.net/addgene:99362 ; RRID:Addgene_99362)

  • For your References section:

    Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on protein stability that destabilise the actin-tropomyosin copolymer in vitro and in vivo. Johnson CA, Brooker HR, Gyamfi I, O'Brien J, Ashley B, Brazier JE, Dean A, Embling J, Grimsey E, Tomlinson AC, Wilson EG, Geeves MA, Mulvihill DP. Biochem Biophys Res Commun. 2017 Oct 25. pii: S0006-291X(17)32087-9. doi: 10.1016/j.bbrc.2017.10.109. 10.1016/j.bbrc.2017.10.109 PubMed 29080743
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