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Addgene

pJC20Cerulean3-Cdc8
(Plasmid #99357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC20
  • Total vector size (bp) 3569
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc8
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    486
  • Entrez Gene
    cdc8 (a.k.a. SPAC27F1.02c, fus4)
  • Promoter T7
  • Tags / Fusion Proteins
    • Cerulean3 (N terminal on insert)
    • His6-Gly3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 terminater
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC20Cerulean3-Cdc8 was a gift from Dan Mulvihill (Addgene plasmid # 99357 ; http://n2t.net/addgene:99357 ; RRID:Addgene_99357)

  • For your References section:

    Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions. Brooker HR, Geeves MA, Mulvihill DP. FEBS Lett. 2016 Sep;590(18):3111-21. doi: 10.1002/1873-3468.12346. Epub 2016 Aug 23. 10.1002/1873-3468.12346 PubMed 27501521
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