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pJC20cdc8
(Plasmid #99354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC20
  • Total vector size (bp) 2803
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc8
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    486
  • Entrez Gene
    cdc8 (a.k.a. SPAC27F1.02c, fus4)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC20cdc8 was a gift from Dan Mulvihill (Addgene plasmid # 99354 ; http://n2t.net/addgene:99354 ; RRID:Addgene_99354)

  • For your References section:

    Acetylation regulates tropomyosin function in the fission yeast Schizosaccharomyces pombe. Skoumpla K, Coulton AT, Lehman W, Geeves MA, Mulvihill DP. J Cell Sci. 2007 May 1;120(Pt 9):1635-45. 10.1242/jcs.001115 PubMed 17452625
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