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HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)
(Plasmid #99342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 6208
  • Modifications to backbone
    introduced second RBS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    STAT1 (710-750)
  • Alt name
    isgf3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    715
  • GenBank ID
    NM_007315
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
  • Promoter T7
  • Tag / Fusion Protein
    • His6GB1 (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CBP (1764-1855)
  • Alt name
    crebbp
  • Species
    M. musculus (mouse)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855) was a gift from Peter Wright (Addgene plasmid # 99342 ; http://n2t.net/addgene:99342 ; RRID:Addgene_99342)

  • For your References section:

    Structural basis for recruitment of CBP/p300 coactivators by STAT1 and STAT2 transactivation domains. Wojciak JM, Martinez-Yamout MA, Dyson HJ, Wright PE. EMBO J. 2009 Apr 8;28(7):948-58. doi: 10.1038/emboj.2009.30. Epub 2009 Feb 12. 10.1038/emboj.2009.30 PubMed 19214187
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