His6tev CBP HAT (1324-1700)
(Plasmid #99340)

• Purpose: bacterial expression of His tagged mouse CBP HAT domain (1324-1700)
• Depositing Lab: Peter Wright
• Publication: Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 10.1073/pnas.1703105114.

Backbone

• Vector backbone: pET22b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5493
• Total vector size (bp): 6714
• Modifications to backbone: introduction of His6tev N-terminal tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mouse CBP HAT domain (1324-1700)
• Alt name: crebbp
• Species: M. musculus (mouse)
• Insert Size (bp): 1221
• Entrez Gene: Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
• Promoter: T7
• Tag / Fusion Protein:
  • His6tev (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry