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Addgene

pET-ZZ(1700-1751)
(Plasmid #99334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 5605
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CBP ZZ domain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    162
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-ZZ(1700-1751) was a gift from Peter Wright (Addgene plasmid # 99334 ; http://n2t.net/addgene:99334 ; RRID:Addgene_99334)

  • For your References section:

    ZZ domain of CBP: an unusual zinc finger fold in a protein interaction module. Legge GB, Martinez-Yamout MA, Hambly DM, Trinh T, Lee BM, Dyson HJ, Wright PE. J Mol Biol. 2004 Oct 29;343(4):1081-93. 10.1016/j.jmb.2004.08.087 PubMed 15476823
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