STARR-seq luciferase INF enhancer vector_ORI_IFI27
(Plasmid
#99320)
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PurposeLuciferase validation vector with IFI27 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSTARR-seq luciferase INF enhancer vector_ORI
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3983
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Vector typeMammalian Expression, Luciferase ; STARR-seq Luciferase validation vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameenhancer; chr14: 94575894-94578286
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SpeciesH. sapiens (human)
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Insert Size (bp)2393
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector has the IFI27 enhancer cloned downstream of the reporter gene; The origin of replication (ORI) substitutes the core promoter . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
STARR-seq luciferase INF enhancer vector_ORI_IFI27 was a gift from Alexander Stark (Addgene plasmid # 99320 ; http://n2t.net/addgene:99320 ; RRID:Addgene_99320) -
For your References section:
Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496
