hSTARR-seq_SCP1 vector_blocking 4
(Plasmid #99319)

• Purpose: (Empty Backbone) Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
• Depositing Lab: Alexander Stark
• Publication: Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.

Backbone

• Vector backbone: STARR-seq luciferase INF enhancer vector_ORI
• Backbone manufacturer: Promega
• Backbone size (bp): 4776
• Vector type: Mammalian Expression ; hSTARR-seq screening vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • hSTARR-seq_SCP1 vector_blocking 4 (seq_CCgoPaK9).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector has a bGH poly A sequence upstream of the ORI , bcl 2 splice acceptor and 4x polyA sequences downstream of the ORI; for blocking transcripts arising from the ORI; Core promoter is SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590