hSTARR-seq_SCP1 vector_blocking 3
(Plasmid
#99318)
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Purpose(Empty Backbone) Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSTARR-seq luciferase INF enhancer vector_ORI
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Backbone manufacturerPromega
- Backbone size (bp) 4457
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Vector typeMammalian Expression ; hSTARR-seq screening vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector has a bcl 2 splice acceptor and 4x polyA sequences downstream of the ORI; for blocking transcripts arising from the ORI; Core promoter is SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hSTARR-seq_SCP1 vector_blocking 3 was a gift from Alexander Stark (Addgene plasmid # 99318 ; http://n2t.net/addgene:99318 ; RRID:Addgene_99318) -
For your References section:
Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496
