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hSTARR-seq_SCP1 vector_blocking 1
(Plasmid #99316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    STARR-seq luciferase INF enhancer vector_ORI
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 4023
  • Vector type
    Mammalian Expression ; hSTARR-seq screening vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector has a RBG polyA and bGH poly A sequence downstream of the ORI; for blocking transcripts arising from the ORI; Core promoter is SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSTARR-seq_SCP1 vector_blocking 1 was a gift from Alexander Stark (Addgene plasmid # 99316 ; http://n2t.net/addgene:99316 ; RRID:Addgene_99316)

  • For your References section:

    Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496
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