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STARR-seq luciferase validation vector_ORI_neg.cont
(Plasmid #99315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    STARR-seq luciferase validation vector_ORI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3977
  • Vector type
    Mammalian Expression, Luciferase ; STARR-seq Luciferase validation vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neutral region; chr3: 55198740-55200041
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1301

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector has a STARR-seq negative region cloned downstream of the reporter gene; The origin of replication (ORI) substitutes the core promoter . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STARR-seq luciferase validation vector_ORI_neg.cont was a gift from Alexander Stark (Addgene plasmid # 99315 ; http://n2t.net/addgene:99315 ; RRID:Addgene_99315)

  • For your References section:

    Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496
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