STARR-seq luciferase validation vector_ORI_SV40
(Plasmid #99309)

• Purpose: Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
• Depositing Lab: Alexander Stark
• Publication: Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.

Backbone

• Vector backbone: STARR-seq luciferase validation vector_ORI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3977
• Vector type: Mammalian Expression, Luciferase ; STARR-seq Luciferase validation vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SV40 enhancer
• Species: H. sapiens (human)
• Insert Size (bp): 237

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • STARR-seq luciferase validation vector_ORI_SV40 (seq_VTJFZMAR).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector has the SV40 enhancer cloned downstream of the reporter gene; The origin of replication (ORI) substitutes the core promoter . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590