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STARR-seq luciferase validation vector_SCP1_empty
(Plasmid #99299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    STARR-seq luciferase validation vector_SCP1_empty
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 4298
  • Vector type
    Mammalian Expression, Luciferase ; STARR-seq Luciferase validation vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector serves as a control for Luciferase assays; there is no enhancer cloned into this construct; Core promoter is the SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STARR-seq luciferase validation vector_SCP1_empty was a gift from Alexander Stark (Addgene plasmid # 99299 ; http://n2t.net/addgene:99299 ; RRID:Addgene_99299)

  • For your References section:

    Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496
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