STARR-seq luciferase validation vector_mP_empty
(Plasmid
#99298)
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Purpose(Empty Backbone) Luciferase validation vector; empty vector without insert to determine baseline
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSTARR-seq luciferase validation vector_mP_empty
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Backbone manufacturerPromega
- Backbone size (bp) 4246
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Vector typeMammalian Expression, Luciferase ; STARR-seq Luciferase validation vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector serves as a control for Luciferase assays; there is no enhancer cloned into this construct; Core promoter is the mP . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
STARR-seq luciferase validation vector_mP_empty was a gift from Alexander Stark (Addgene plasmid # 99298 ; http://n2t.net/addgene:99298 ; RRID:Addgene_99298) -
For your References section:
Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496
