hSTARR-seq_ORI vector
(Plasmid #99296)

• Purpose: (Empty Backbone) Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
• Depositing Lab: Alexander Stark
• Publication: Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.

Backbone

• Vector backbone: hSTARR-seq_ORI vector
• Backbone manufacturer: Promega
• Backbone size (bp): 2537
• Vector type: Mammalian Expression ; hSTARR-seq screening vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • hSTARR-seq_ORI vector (seq_Pp7FEhGh).gb
• Addgene Notes:
  • Addgene Diagnostic Digest -SalI
• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech). In this vector the origin of replication (ORI) substitutes a core promoter . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590