hSTARR-seq_TTF2 vector
(Plasmid
#99293)
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Purpose(Empty Backbone) Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonehSTARR-seq_TTF2 vector
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Backbone manufacturerPromega
- Backbone size (bp) 2857
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Vector typeMammalian Expression ; hSTARR-seq screening vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hSTARR-seq_TTF2 vector was a gift from Alexander Stark (Addgene plasmid # 99293 ; http://n2t.net/addgene:99293 ; RRID:Addgene_99293) -
For your References section:
Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496