pCW57.1-Luciferase
(Plasmid #99283)

• Purpose: All-in-one lentivector with tet-inducible luciferase
• Depositing Lab: Stephen Tapscott
• Publication: Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. doi: 10.1371/journal.pgen.1006658. eCollection 2017 Mar.

Backbone

• Vector backbone: pCW57.1
• Backbone size w/o insert (bp): 9354
• Total vector size (bp): 9462
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: We used Stbl3 cells (recombination deficient)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly luciferase
• Species: Firefly
• Insert Size (bp): 1653
• Promoter: TRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: tcgcctggagaattggc
• 3′ sequencing primer: agggctgccttggaaaag

Resource Information

• Supplemental Documents:
  • pCW57.1_Luciferase.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry