pCW57.1-DUX4-WT
(Plasmid #99282)

• Purpose: All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)
• Depositing Lab: Stephen Tapscott
• Publication: Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4431. doi: 10.1093/hmg/ddw271.

Backbone

• Vector backbone: pCW57.1
• Backbone size w/o insert (bp): 9354
• Total vector size (bp): 9132
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: We used Stbl3 cells (recombination deficient)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DUX4
• Species: H. sapiens (human)
• Insert Size (bp): 1275
• Entrez Gene: DUX4 (a.k.a. DUX4L)
• Promoter: TRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: tcgcctggagaattggc
• 3′ sequencing primer: agggctgccttggaaaag

Resource Information

• Supplemental Documents:
  • pCW57.1-DUX4_mal103.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry