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Addgene

pAdCMV/V5-DEST-S727A-STAT3-3xFlag
(Plasmid #99262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAdCMV/V5-DEST
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 34621
  • Total vector size (bp) 36945
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Signal transducer and activator of transcription 3 - S727A
  • Alt name
    S727A-STAT3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2324
  • Mutation
    S727A
  • Entrez Gene
    Stat3 (a.k.a. 1110034C02Rik, Aprf)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x-Flag (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    3xFlag from Addgene plasmid #20754 and STAT3 from Addgene plasmid #8707

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*E16K mutation in STAT3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdCMV/V5-DEST-S727A-STAT3-3xFlag was a gift from Alejandro Adam (Addgene plasmid # 99262 ; http://n2t.net/addgene:99262 ; RRID:Addgene_99262)

  • For your References section:

    Interleukin-6 promotes a sustained loss of endothelial barrier function via Janus kinase-mediated STAT3 phosphorylation and de novo protein synthesis. Alsaffar H, Martino N, Garrett JP, Adam AP. Am J Physiol Cell Physiol. 2018 Jan 10. doi: 10.1152/ajpcell.00235.2017. 10.1152/ajpcell.00235.2017 PubMed 29351406
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