pGLACTE-HT51-10
(Plasmid #99255)

• Purpose: Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. To generate capillary electrophoresis size standards.
• Depositing Lab: Karen Usdin
• Publication: Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi: 10.1016/j.jmoldx.2017.06.008. Epub 2017 Aug 14.

Backbone

• Vector backbone: pGL3
• Total vector size (bp): 4945

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FMR1
• Species: H. sapiens (human)
• Insert Size (bp): 554
• Entrez Gene: FMR1 (a.k.a. FMRP, FRAXA, POF, POF1)
• Tag / Fusion Protein:
  • eGFP

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATAAGCTTTAGGCGTGTACGG
• 3′ sequencing primer: cgctgaacttgtggccgttta

Resource Information

• Supplemental Documents:
  • SnapGene annotation file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CGG repeats are moderately unstable in prolonged bacterial culture at 37C.
Upon receipt select several colonies, grow up overnight and immediately use to make glycerol stocks and verify insert size.
For large scale preparations inoculate directly from the glycerol stock and culture for ~10 hours.