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Purpose(Empty Backbone) single-vector system with EGFP reporter for use in CROPseq knockout screens
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCROPseq-Guide-Puro
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Backbone manufacturerBock lab
- Backbone size (bp) 10214
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Modifications to backboneremoved replaced EF1a promoter with EFS promoter. Removed Puromycin resistant gene and added SpCas9-P2A-EGFP
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter EFS
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byconstruct cloned using CROPseq-Guide-Puro vector as backbone (Addgene ID 86708)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROPseq-Guide-EFS-SpCas9-P2A-EGFP was a gift from Alex Hewitt (Addgene plasmid # 99248 ; http://n2t.net/addgene:99248 ; RRID:Addgene_99248)
Map uploaded by the depositor.
