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PurposeLacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 99247 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backboneno vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BL21-Gold-dLac (DE3)
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Copy numberUnknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The strain is used for producing bacterial lysates with no beta-galactosidase activity.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain was a gift from Jeff Hasty (Addgene plasmid # 99247) -
For your References section:
Rapid and scalable preparation of bacterial lysates for cell-free gene expression. Didovyk A, Tonooka T, Tsimring L, Hasty J. ACS Synth Biol. 2017 Aug 10. doi: 10.1021/acssynbio.7b00253. 10.1021/acssynbio.7b00253 PubMed 28795570