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pCI-Neo-p53mut
(Plasmid #99239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 6642
  • Modifications to backbone
    p53 cDNA inserted in MCS of vector between EcoRI and XbaI sites. A Kozak sequence was for enhanced translation initiation.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Alt name
    TP53
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1170
  • Mutation
    Silent mutations in translated protein at Gly (103) Ser and Glu (256) Gly.
  • GenBank ID
    U90328
  • Entrez Gene
    Tp53 (a.k.a. Trp53, p53)
  • Promoter CMV immediate/early enhancer/promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcorRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAG
  • 3′ sequencing primer ATTAACCCTCACTAAAGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's QC result finds an additional K318E mutation, which the depositor notes should have no functional consequence as it is away from the dimerization and DNA binding motifs

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo-p53mut was a gift from Peter Pedersen (Addgene plasmid # 99239 ; http://n2t.net/addgene:99239 ; RRID:Addgene_99239)

  • For your References section:

    Glucose catabolism in cancer cells. The type II hexokinase promoter contains functionally active response elements for the tumor suppressor p53. Mathupala SP, Heese C, Pedersen PL. J Biol Chem. 1997 Sep 5;272(36):22776-80. PubMed 9278438
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