pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
(Plasmid #99229)

• Purpose: Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.
• Depositing Lab: Periklis Pantazis
• Publication: Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 4012
• Total vector size (bp): 4793
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pr-mEos2
• Alt name: prmEos2, mEos2-A69T
• Species: Synthetic
• Insert Size (bp): 681
• Mutation: A69T for primed conversion applications
• Promoter: CMV
• Tag / Fusion Protein:
  • Lifeact (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: GAAATTTGTGATGCTATTGC

Resource Information

• Supplemental Documents:
  • mEos2-A69T-Lifeact-7.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry