pRSET-pr-Kaede (Kaede-A69T)
(Plasmid #99228)

• Purpose: Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).
• Depositing Lab: Periklis Pantazis
• Publication: Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121.

Backbone

• Vector backbone: pRSET
• Backbone size w/o insert (bp): 2860
• Total vector size (bp): 3541
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pr-Kaede
• Alt name: prKaede, Kaede-A69T
• Species: Trachyphyllia geoffroyi
• Insert Size (bp): 681
• Mutation: A69T for primed conversion applications
• GenBank ID: AB085641
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: GAGCGAGGAAGCGGAAGAG
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pRSETb-Kaede-A69T.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry